What is TAE Buffer used for in molecular biology?

TAE Buffer, or Tris-Acetate-EDTA, is primarily used in molecular biology for the electrophoresis of nucleic acids such as DNA and RNA. It provides a stable pH environment, ensuring that nucleic acids maintain their integrity during the electrophoresis process. Additionally, EDTA chelates divalent metal ions, which can otherwise cause degradation of nucleic acids. It is essential in applications such as gel electrophoresis, cloning, and sequencing, making it a versatile reagent in laboratories.

How do I prepare 50x TAE Buffer from concentrate?

To prepare 50x TAE Buffer from concentrate, you'll need to dilute it by adding distilled water. Mix 1 part of the concentrated buffer with 49 parts distilled water to create a working solution. This concentrated buffer is typically used for making agarose gels, where the 1x TAE is applied. Preparing the buffer correctly ensures optimal results in gel electrophoresis, promoting effective separation of DNA fragments based on size.

What is the shelf life of TAE Buffer once opened?

Once opened, TAE Buffer can remain usable for several months if stored properly, away from light and at room temperature. It's advisable to check for any changes in color or precipitate formation before use. Ensuring good laboratory practices, such as using clean pipette tips, can help maintain the integrity of the buffer, allowing it to function effectively in electrophoresis experiments. Always label bottles with the date opened for reference.

Can I use TAE Buffer for RNA analysis?

Yes, TAE Buffer is suitable for both DNA and RNA analysis. It provides an excellent medium for RNA electrophoresis, maintaining the stability and integrity of RNA molecules. The buffer helps minimize RNA degradation, making it ideal for techniques like Northern blotting. When working with RNA, it's essential to take precautions against RNases by using clean equipment and reagents to prevent contamination.

What are the advantages of using 50x TAE Buffer over other buffers?

50x TAE Buffer offers several advantages compared to alternative buffers like TBE. It has a lower ionic strength, which results in improved resolution of larger DNA fragments during electrophoresis. Additionally, TAE is less likely to cause precipitation in gel-making, making it a preferred choice for many protocols. Its compatibility with a variety of applications, such as cloning and PCR, also adds to its utility in the laboratory.

Is TAE Buffer safe to handle in the lab?

TAE Buffer is generally considered safe to handle, as its components are non-toxic and do not pose significant health risks. However, as with any laboratory chemical, it's advisable to follow standard safety precautions. This includes wearing gloves and eye protection, and working in a well-ventilated area. Proper disposal of waste containing TAE Buffer should also be adhered to according to your institution's guidelines, ensuring a safe laboratory environment.

How do I store TAE Buffer for long-term use?

For long-term storage, it is best to keep TAE Buffer in a cool, dark place. Storing it in a 4°C refrigerator can help prolong its shelf life. It's essential to ensure that the container is tightly sealed to prevent contamination and evaporation. Consider aliquoting the buffer into smaller containers, which allows you to reduce the number of times the entire stock is opened, minimizing exposure to potential contaminants.

What is the pH of TAE Buffer and why is it important?

TAE Buffer has a pH of approximately 8.0, which is crucial for maintaining the stability of nucleic acids during electrophoresis. This specific pH allows for optimal migration of DNA and RNA, ensuring reliable and reproducible results. The balanced pH also prevents nucleic acid denaturation, making it particularly important in applications where integrity is vital, such as in cloning procedures and molecular characterizations.

Can TAE Buffer be reused after gel electrophoresis?

While TAE Buffer can technically be reused after gel electrophoresis, it is generally not recommended. Reusing buffer can lead to contamination and accumulation of ionic impurities, which may affect the resolution of subsequent electrophoresis runs. If you choose to reuse the buffer, it's advisable to filter it to remove any cellular debris and monitor its conductivity and pH to ensure it remains suitable for further use.

Where can I buy 50x TAE Buffer (10 L) in Hong Kong?

You can purchase 50x TAE Buffer (10 L) from Ubuy, which offers a variety of laboratory supplies available for shipping. Ubuy provides a reliable platform for sourcing quality products tailored to meet your laboratory needs, ensuring that you have access to essential reagents like TAE Buffer efficiently.

如何從 Ubuy 在線購物 50x TAE Buffer (10 L)？

從 Ubuy 在線購物 50x TAE Buffer (10 L) 非常簡單。. 您只需搜索產品，在結賬時選擇運輸方式，然後將其運送到您所在的位置。

50x TAE Buffer (10 L) 可以在 Hong Kong 在線購物嗎？

是的，您可以在 Ubuy Hong Kong 以合理的價格購買該產品。. 50x TAE Buffer (10 L) 在本地不可用，但您可以信任我們的快遞服務。

下訂單後需要多長時間才能收到產品？

您訂購的產品的交貨時間根據您訂購的商品和您選擇的運輸方式而有所不同。. 結賬時會提到預計送貨時間，所以購物時請放心。

觀看原文

50X TAE Buffer is essential for achieving optimal resolution of RNA and DNA fragments larger than 1500 bp.

物品 #: 125663516

50x TAE Buffer (10 L)

94% of respondents would recommend this to a friend

物品 #: 125663516

HKD 2575

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

^{*All items will import from 美國}

供應情形 : 有存貨

從USA商店導入

QTY:

現在訂購，約可在下列時間收貨：星期六, 十月 17

Secured transaction

Our Top Logistics Partners

50X TAE Buffer is essential for achieving optimal resolution of RNA and DNA fragments larger than 1500 bp.

U-Care Warranty:

None

選擇一個計劃

Fast
Shipping

Free
Return^*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

Note: Step Down Voltage Transformer required for using electronics products of 美國 store (110-120). Recommended power converters 立即購買.

What Stands Out

Concentrated Solution

This 50x TAE buffer offers a highly concentrated formula, requiring only dilution for easy preparation of electrophoresis experiments, saving time and ensuring consistent results.

Versatile Applications

Ideal for a variety of molecular biology applications, this TAE buffer supports DNA and RNA electrophoresis, catering to the diverse needs of researchers and labs.

Cost-Effective Bulk

The 10-liter size provides exceptional value for laboratories, reducing the frequency of purchases while ensuring ample supply for extensive experimental needs.

產品詳情

Brand Name	Generic
Manufacturer	Bioland Scientific LLC
Included Components	buffer
Model Number	TAE01-10L
Part Number	TAE01-10L
Item Weight	22 lbs (9.98 kg)

Who Should Buy?

Molecular Biologists
Ideal for molecular biology experiments requiring TAE buffer for DNA gel electrophoresis and nucleic acid stabilization.
Educational Institutions
Perfect for schools and universities conducting laboratory courses involving DNA analysis and gel electrophoresis experiments.
Research Laboratories
Suitable for research labs that frequently analyze nucleic acids and need a reliable buffer for various experiments.

Home Users
Not intended for casual or home-based experiments that do not require a bulk supply of laboratory buffers.

產品敘述

50x TAE Buffer (10 L)

有疑問？與我們聊天

客戶問題與解答

題: 如何從 Ubuy 在線購物 50x TAE Buffer (10 L)？

回答: 從 Ubuy 在線購物 50x TAE Buffer (10 L) 非常簡單。. 您只需搜索產品，在結賬時選擇運輸方式，然後將其運送到您所在的位置。
題: 50x TAE Buffer (10 L) 可以在 Hong Kong 在線購物嗎？

回答: 是的，您可以在 Ubuy Hong Kong 以合理的價格購買該產品。. 50x TAE Buffer (10 L) 在本地不可用，但您可以信任我們的快遞服務。
題: 下訂單後需要多長時間才能收到產品？

回答: 您訂購的產品的交貨時間根據您訂購的商品和您選擇的運輸方式而有所不同。. 結賬時會提到預計送貨時間，所以購物時請放心。

Buffers TAE01-10L Editorial Review

50x TAE Buffer (10 L) is a high-quality buffer solution designed for use in molecular biology applications. Manufactured by Bioland Scientific LLC, this buffer is crucial for DNA electrophoresis, offering reliable performance. At 22 lbs, it comes in a convenient bulk size with a model number TAE01-10L, ensuring that you have ample supply for your experiments. Users appreciate its consistent quality and its pivotal role in achieving accurate and repeatable results in their gel applications.

Customer Reviews & Ratings

5.0

1 客戶評分

5 星

100%
4 星

0%
3 星

0%
2 星

0%
1 星

0%

評價此產品

與其他客戶分享您的想法

優點

High-quality buffer for molecular biology applications
Reliable performance for DNA electrophoresis
Convenient bulk size for prolonged use
consistent results in gel applications
Manufactured by a trusted company

缺點

Some might find the weight a bit heavy

Product Price History

重要資訊

限制：如跨國購買產品，請注意製造商的保固有可能無效；製造商服務選項可能無法使用；產品手冊、教學、以及安全警示可能不會是目的地國家的語言；產品（與附加材料）設計也許不會符合目的地國家的標準、規格、以及標示要求；且產品也許不會符合目的地國家的伏特數值與其他電力標準（需要使用合適的變壓器或轉接器）。收件者須負責確定該產品在目的地國家可合法進口。當在 Ubuy 或其聯盟夥伴網站上訂購時，收件者為記錄上的進口者，且必須遵從目的地國家的法律與規範。
由於 Ubuy 是一個全球搜尋引擎，並不是所有列於 Ubuy 的產品都有出售。產品須受出口／貿易法規規範。

HKD 2575

現在訂購，約可在下列時間收貨：星期六, 十月 17

QTY:

Secured transaction

PCI DSS compliant and ISO 27001:2022 certified, with encrypted payments and full buyer protection on every order.

Our Top Logistics Partners

Fastest cross-border delivery

特色和優勢

Ideal for electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Can be used as both running and gel preparation buffer.
Filters through a 0.22 µm membrane for purity.
Recommended for genomic and large supercoiled DNA.
Ensures high resolution for fragments larger than 1500 bp.
Easy to use and mix for reliable lab results.

Ubuy Assurance

Experience worry-free shopping with 100% original products, PCI DSS-compliant payment security, ISO 27001-certified data protection, the fastest cross-border delivery, free returns ^*, and secure packaging on every order.

50x TAE Buffer (10 L)

HKD 2575

Fast Shipping

Free Return*

Secure Packaging

100% Original Products

PCI DSS Compliance

ISO 27001 Certified

What Stands Out

產品詳情

Who Should Buy?

產品敘述

客戶問題與解答

Buffers TAE01-10L Editorial Review

Customer Reviews & Ratings

評價此產品

優點

缺點

Product Price History

重要資訊

HKD 2575

特色和優勢

Ubuy Assurance

Fast
Shipping

Free
Return^*